Lentiviral Production Co-Op
Company: Legend Biotech
Location: Philadelphia
Posted on: February 25, 2026
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Job Description:
Legend Biotech is a global biotechnology company dedicated to
treating, and one day curing, life-threatening diseases.
Headquartered in Somerset, New Jersey, we are developing advanced
cell therapies across a diverse array of technology platforms,
including autologous and allogenic chimeric antigen receptor
T-cell, T-cell receptor (TCR-T), and natural killer (NK) cell-based
immunotherapy. From our three R&D sites around the world, we
apply these innovative technologies to pursue the discovery of
safe, efficacious and cutting-edge therapeutics for patients
worldwide. Legend Biotech entered into a global collaboration
agreement with Janssen, one of the pharmaceutical companies of
Johnson & Johnson, to jointly develop and commercialize
ciltacabtagene autolecuel (cilta-cel). Our strategic partnership is
designed to combine the strengths and expertise of both companies
to advance the promise of an immunotherapy in the treatment of
multiple myeloma. Legend Biotech is seeking Lentiviral Production
Co-Op as part of the R&D team based in Philadelphia, PA . Role
Overview The Lentiviral Production Co-op will support upstream and
downstream processes for lentiviral vector manufacturing, including
cell culture, transfection, purification, and analytical testing in
a BSL-2 environment. This hands-on role offers exposure to
industry-standard techniques and equipment while collaborating with
scientists and engineers to optimize workflows for gene therapy
applications. Co-Op Duration : 6-months (Summer to Fall) with
possible extension Key Responsibilities Assist in culturing
suspension and adherent HEK293 cells Support lentiviral
transfection and harvest process, including media preparation and
monitoring cell growth and viability Perform buffer preparation,
system setup, and cleaning following established SOPs. Operate AKTA
chromatography systems for purification steps (ion-exchange and/or
affinity) Perform Tangential flow filtration (TFF) for
concentration and buffer exchange of lentiviral vectors Monitor
process parameters and troubleshoot basic issues under supervision
Assist in formulation, sterile filtration, and aliquoting of
purified lentiviral products Perform lentiviral tittering using
functional assays (TU/mL) and p24 ELISA for capsid quantification
Conduct endotoxin testing to ensure product safety and compliance
with regulatory standards Collect, analyze, and document process
data to support optimization studies. Maintain accurate laboratory
records and ensure compliance with safety and quality standards.
Collaborate with scientists and engineers to troubleshoot and
improve purification workflows. Requirements Currently enrolled in
Master’s program in Biotechnology, Biochemical engineering,
molecular biology, or related field. Basic understanding of cell
culture, virology, or bioprocessing principles preferred.
Familiarity with laboratory techniques such as pipetting, buffer
preparation, and aseptic handling. Familiarity with chromatography
systems (AKTA) and TFF is a plus. Comfortable working in a BSL-2
environment. Strong attention to detail, organizational skills, and
ability to work independently and in a team environment. Excellent
communication and documentation skills. What we offer: Hands-on
experience in viral vector production processes such as upstream,
downstream, and analytics. Opportunity to work in a collaborative
and innovative environment. Exposure to industry-standard equipment
and processes in gene therapy manufacturing. Li-JR1 Li-Onsite
Please note: These benefits are offered exclusively to permanent
full-time employees. Contract employees are not eligible for
benefits through Legend Biotech. EEO Statement It is the policy of
Legend Biotech to provide equal employment opportunities without
regard to actual or perceived race, color, creed, religion,
national origin, ancestry, citizenship status, age, sex or gender
(including pregnancy, childbirth, related medical conditions and
lactation), gender identity or gender expression (including
transgender status), sexual orientation, marital status, military
service and veteran status, disability, genetic information, or any
other protected characteristic under applicable federal, state or
local laws or ordinances. Employment is at-will and may be
terminated at any time with or without cause or notice by the
employee or the company. For information related to our privacy
policy, please review: Legend Biotech Privacy Policy.
Keywords: Legend Biotech, Philadelphia , Lentiviral Production Co-Op, Science, Research & Development , Philadelphia, Pennsylvania
